Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58.

نویسندگان

  • Khomaini Hasan
  • Andrea Fortova
  • Tana Koudelakova
  • Radka Chaloupkova
  • Mayuko Ishitsuka
  • Yuji Nagata
  • Jiri Damborsky
  • Zbynek Prokop
چکیده

We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 77 5  شماره 

صفحات  -

تاریخ انتشار 2011